PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.

PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.

The examine of protein-RNA interactions is essential for our understanding of mobile processes and regulatory circuits managed by RNA binding proteins (RBPs). Current subsequent era sequencing-based approaches considerably promoted our understanding of RNA biology and its significance for cell perform. We current a streamlined protocol for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a method that permits […]

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Identification of immune protective genes of Eimeria maxima through cDNA expression library screening.

Identification of immune protective genes of Eimeria maxima through cDNA expression library screening.

Eimeria maxima is among the most prevalent Eimeria species inflicting avian coccidiosis, and leads to enormous financial loss to the worldwide poultry business. Present management methods, resembling anti-coccidial remedy and dwell vaccines have been restricted due to their drawbacks. The third technology anticoccidial vaccines together with the recombinant vaccines in addition to DNA vaccines have […]

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Transcriptome analysis of leaf tissue from Bermudagrass (Cynodon dactylon) using a normalised cDNA library

Transcriptome analysis of leaf tissue from Bermudagrass (Cynodon dactylon) using a normalised cDNA library

A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodon dactylon L. A complete of 15 588 high-quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to provide a unigene set. A complete of 9414 unigenes […]

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Ultrasensitive multiplexed detection of miRNA targets of interest based on encoding probe extension in improved cDNA library

Ultrasensitive multiplexed detection of miRNA targets of interest based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play essential roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a particular most cancers is related to expression alterations of a number of species of miRNAs and various kinds […]

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