PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.

PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.

The examine of protein-RNA interactions is essential for our understanding of mobile processes and regulatory circuits managed by RNA binding proteins (RBPs). Current subsequent era sequencing-based approaches considerably promoted our understanding of RNA biology and its significance for cell perform. We current a streamlined protocol for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a method that permits […]

Continue Reading